Journal: Diabetes & Metabolism Journal

Article Title: Effects of CXCR1/2 Blockade with Ladarixin on Streptozotocin-Induced Type 1 Diabetes Mellitus and Peripheral Neuropathy and Retinopathy in Rat

doi: 10.4093/dmj.2024.0504

Figure Lengend Snippet: Late ladarixin (LDX) normalized signs of streptozotocin (STZ)-induced diabetic retinopathy and diabetic nephropathy: retinal expression of CXC motif chemokine ligand 8 (CXCL8), CXC motif chemokine receptor 1 (CXCR1), citrullinated histone H3 (CitH3) myeloperoxidase (MPO), CD34, and vascular endothelial growth factor (VEGF) and of plasma creatinine levels in STZ-treated rats. Effects of LDX (15 mg/kg, intragastric [i.g.]) treatment (from week 8 to 12) in control (CTRL), STZ/vehicle (VEH), and STZ/LDX groups on the representative images of (A, H) CitH3, (B, I) MPO, (C, J) CXCL8, (D, K) CXCR1, and (E, L) CXCR2 expression in the retina layers and relative quantification measured as fluorescence intensity per area at different time points post-STZ injection. Effects of LDX (15 mg/kg, i.g.) treatment (from week 8 to 12) in CTRL, STZ/VEH, and STZ/LDX groups on the representative images of (F, M) CD34 and (G, N) VEGF expression in the retina layers and relative quantification measured as fluorescence intensity per area at different time points post-STZ injection. Data are represented as mean±standard error of the mean ( n =3). (O) Effects of LDX (15 mg/kg, i.g.) treatment (from week 8 to 12) in CTRL, STZ/VEH, and STZ/LDX groups on creatinine (pg/mL) measured at 9- and 12-week post-STZ injection. Data are represented as mean±standard error of the mean ( n =4). The threshold of 0.05 was used for determining statistical significance. The magnification of immunofluorescence picture is of ×20. GRO/CINC1, growth-regulated gene product/cytokine-induced neutrophil chemoattractant 1. a P <0.0001, b P <0.001, and c P <0.0001 indicate significant differences vs. CTRL and STZ/VEH.

Article Snippet: Likewise, the eyes sections with antibodies against histone H3 (rabbit anti-histone H3 antibody; 1:200; Abcam), MPO (mouse anti-MPO antibody; 1:50; Abcam), CXCL1 (mouse CXCL8 monoclonal antibody; 1:400; Thermo Fisher Scientific, Waltham, MA, USA), CXCR1 (rabbit CXCR1 polyclonal antibody; 1:100; Elabscience, Houston, TX, USA), CXCR2 (rabbit CXCR2 polyclonal antibody; 1:100; Elabscience), VEGF (rabbit VEGF recombinant rabbit monoclonal antibody; 1:200; Thermo Fisher Scientific), and CD34 (rabbit CD34 polyclonal antibody; 1:100; Elabscience).

Techniques: Expressing, Clinical Proteomics, Control, Quantitative Proteomics, Fluorescence, Injection, Immunofluorescence

Journal: International Journal of Nanomedicine

Article Title: Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model

doi: 10.2147/IJN.S398446

Figure Lengend Snippet: The characteristics of macrophages and BMSCs. ( a ) Primary macrophages observed under the microscope; cells were spherical and uniform in size. ( b ) Flow cytometry results of CD68 expression on the cell surface. The expression of CD68 on 10,000 cells was recorded. 90.7% of the cells expressed CD68. ( c ) The fifth generation of cultured BMSCs was observed under the microscope; the cells were long, fusiform, and translucent. ( d – f ) Alizarin red, Alcian blue, and Oil red O staining showed that the cells had osteogenic, chondrogenic, and adipogenic differentiation ability. ( g – j ). The expression of CD29, CD90, CD44, and CD34 on 10,000 cells was recorded; red indicates cell marker expression and blue indicates the isotype control. Microscopy: 100× magnification and 200 μm scale.

Article Snippet: Cells were incubated with the following specific antibodies in the dark at 4 °C for 30 min: rabbit anti-CD29/Alexa Fluor 488 (1:100 dilution), rabbit anti-CD90/Alexa Fluor 488 (1:100 dilution), rabbit anti-CD44/Alexa Fluor 488 (1:100 dilution), rabbit anti-CD34/Alexa Fluor 488 (1:100 dilution), and rabbit anti-CD68/Alexa Fluor 488 (1:100 dilution) (all purchased from Bioss, China).

Techniques: Microscopy, Flow Cytometry, Expressing, Cell Culture, Staining, Marker

Journal: Frontiers in Pharmacology

Article Title: Mangiferin mitigates dexamethasone-induced insulin resistance in rats: insight into vascular dysfunction and hepatic steatosis

doi: 10.3389/fphar.2025.1572758

Figure Lengend Snippet: Impact of Mang on the aortic expression of CD34 and VEGF: Panel (I) : Photomicrographs of CD34-immune stained sections in the aorta: (A) CTRL group: showing strong positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads). (B,C) DEX-treated group: showing weak positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads) compared to the CTRL group. (D) Mang (25 mg/kg)-pretreated group: showing mild positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads) but more than the DEX-treated group. (E) Mang (50 mg/kg)-pretreated group: showing moderate positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads). (F) Mang (100 mg/kg)-pretreated group: showing strong positive CD34 immunostaining confined to the cytoplasm of endothelial cells (arrowheads) more or less similar to the CTRL group. Panel (II) : Photomicrographs of VEGF-stained sections in the aorta: (A) CTRL group: showing minimal VEGF immunostaining in the cytoplasm of endothelial cells (arrowhead). (B,C) DEX-treated group: showing more abundant positive VEGF immunostaining in the cytoplasm of endothelial cells (arrowheads) and in vascular smooth muscles (curved arrows) more than CTRL group. (D) Mang (25 mg/kg)-pretreated group: showing strong positive VEGF immunostaining in the cytoplasm of endothelial cells (arrowheads) and in vascular smooth muscles (curved arrows) but less than the DEX-treated group. (E) Mang (50 mg/kg)-pretreated group: showing moderate positive VEGF immunostaining confined to the cytoplasm of endothelial cells (arrowheads). (F) Mang (100 mg/kg)-pretreated group: showing scarce positive VEGF immunostaining confined to the cytoplasm of endothelial cells (arrowheads) more or less similar to the CTRL group. Image magnification = ×400. CTRL: control; DEX: dexamethasone; Mang: mangiferin; VEGF: vascular endothelial growth factor.

Article Snippet: Polyclonal antibodies were obtained from Thermo-Fisher Scientific Anatomical Pathology, California, United States, including TNF-α; Cat No. PA1-40281, IRS1; PA5-104876, eNOS; PA1-037, CD34; MA5-35202, respectively, and VEGF antibodies (Cat No. bs-0279R, Bioss, Inc., Woburn, Massachusetts, United States) for utilization in the Biotin-Avidin complex method ( ).

Techniques: Expressing, Staining, Immunostaining, Muscles, Control

Journal: Frontiers in Pharmacology

Article Title: Mangiferin mitigates dexamethasone-induced insulin resistance in rats: insight into vascular dysfunction and hepatic steatosis

doi: 10.3389/fphar.2025.1572758

Figure Lengend Snippet: Schematic illustration of the protective mechanism of Mang against DEX-induced IR in rats. AKT: protein kinase B; ALT: alanine aminotransferase; AMPK: AMP-activated protein kinase protein kinase; AST: aspartate aminotransferase; DEX: dexamethasone; eNOS: endothelial nitric oxide synthase; EPCs: endothelial progenitor cells; ET-1: endothelin-1; FFA: free fatty acids; FOXO-1: forkhead box protein O1; FSI: fasting serum insulin; G6Pase: glucose 6-phosphatase; GS: glycogen synthase; GSH: reduced glutathione; GSK-3 glycogen synthase kinase 3; HDL-C: high-density density lipoprotein; IRS1: insulin receptor substrate-1; JNK: C-Jun N-terminal kinases; LDH: lactate dehydrogenase; LDL-C: low-density density lipoprotein; Mang: mangiferin; MDA: malondialdehyde; NF-κB: nuclear factor-kappa B; NLRP3: NOD-like receptor family pyrin domain-containing 3; OGTT: oral glucose tolerance test; PEPCK: phospho-enol pyruvate carboxy kinase; PGI2: prostacyclin; PPAR-γ: peroxisome proliferator-activated receptor-γ; ROS: reactive oxygen species; SOD: superoxide dismutase; TC: total cholesterol; TG: triglycerides; TNF-α: tumor necrosis factor alpha; VCAM: vascular cell adhesion molecule; VEGF: vascular endothelial growth factor; VLDL-C: very-low-density lipoprotein.

Article Snippet: Polyclonal antibodies were obtained from Thermo-Fisher Scientific Anatomical Pathology, California, United States, including TNF-α; Cat No. PA1-40281, IRS1; PA5-104876, eNOS; PA1-037, CD34; MA5-35202, respectively, and VEGF antibodies (Cat No. bs-0279R, Bioss, Inc., Woburn, Massachusetts, United States) for utilization in the Biotin-Avidin complex method ( ).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Comparative study on the biological characteristics of menstrual blood- and endometrium-derived endometrial cells

doi: 10.3892/etm.2021.10856

Figure Lengend Snippet: Detection of cell markers by immunofluorescence staining and flow cytometry. (A) MB-ECs and E-ECs expressed CK18. (B) MB-ECs and E-ECs did not express Vim. (C) MB-ECs and E-ECs did not express CD34 and CD90. Scale bar=100 µm. CK18, cytokeratin 18; Vim, vimentin; NC, negative control; ECs, endometrial cells; MB, menstrual blood-derived; E, endometrium-derived.

Article Snippet: Following digestion into single cells with warmed 0.25% trypsin-EDTA, MB-ECs and E-ECs were incubated with anti-CD90-PE (1:200; cat. no. bs-10430R-PE; BIOSS), anti-CD34-PE (1:200; cat. no. bs-0646R-PE; BIOSS), unconjugated antibodies or matched-isotype control IgG (1:200; cat. no. bs-0295P-PE; BIOSS) for 1 h at room temperature and analyzed via flow cytometry using a BD Accuri ™ C6 flow cytometer (BD Biosciences).

Techniques: Immunofluorescence, Staining, Flow Cytometry, Negative Control, Derivative Assay

Journal: PLoS ONE

Article Title: Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes

doi: 10.1371/journal.pone.0204677

Figure Lengend Snippet: (A) Phase contrast image of bladder SMCs at passage 3. (B) Phase contrast images of EPCs at passages 0 and 3, respectively. (C) Tubular network formation of EPCs on Matrigel. (D) EPCs were identified by ac-LDL endocytosis and UEA-1 binding. Nuclei were stained with DAPI. (E) Immunofluorescence images showing CD34 and VEGFR2 expression in EPCs. Nuclei were stained with DAPI. The scale bars show 100 μm in (A), 200 μm in (B, C), and 50 μm in (D, E).

Article Snippet: In addition, immunofluorescence assay was performed using RBITC conjugated anti-CD34 and FITC conjugated anti-vascular endothelial growth factor receptor 2 (VEGFR2) polyclonal antibodies (Bioss, China).

Techniques: Binding Assay, Staining, Immunofluorescence, Expressing